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The increased use of hospital isolation rooms due to emerging infectious diseases has raised concerns about air containment failure through the passage of staff, especially with hinged doors. Preventing unintended airflow leakage during door movements is crucial. Instead of costly and labor-intensive experimental measurements, Computational Fluid Dynamics (CFD) modeling with time-resolved Large Eddy Simulation (LES) offers an effective alternative. This study compared simulated airflow patterns and total air volume escaping with experimental data. The LES method successfully replicated complex transient airflows during door motions and human passage between rooms. While this initial test was in an isothermal environment without ventilation, the CFD approach can easily accommodate additional factors. Graphical methods for simulating smoke experiments through LES were introduced. The results highlight the potential of transient CFD simulation to evaluate isolation room scenarios, mimicking airflow complexities and estimating air escape volumes over time. The calculated air volume differed by 20% from experimental measurements in this study, demonstrating the effectiveness of the approach. The images and data used here are adapted from the work of: Pekka E. Saarinen ,Petri Kalliomäki,Julian W. Tang,Hannu Koskela in the article titled '[Large Eddy Simulation of Air Escape through a Hospital Isolation Room Single Hinged Doorway—Validation by Using Tracer Gases and Simulated Smoke Videos]' published in the journal [PLOS ONE]. The source can be found at [Link to the data source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130667] . The images and data are licensed under CC BY 4.0 [License Link: https://creativecommons.org/licenses/by/4.0/]."